ChemBioSys I

We investigated the role of chemical signaling between two co-occurring bacteria that each make up more than 10% of the community in iron-rich lakes aggregates (iron snow) by comparative metagenomics and propose a model that shows rapid iron snow formation and ultimately energy transfer from the photic zone to deeper water layers are controlled via a chemically mediated interplay.

We study the transcriptional landscape of C. glabrata and identify differentially expressed genes between pH4 and ph8 as well as between nitrosative stress (GSNO) treatment and controll. Candida glabrata was grown in standard nutrient-rich medium, ph4, ph8 and in GSNO conditions. Paired-end and strand-specific RNA-Seq was performed on three biological replicates after PolyA filtering. A fourth replicate without filtering was sequenced single-end and not strand-specific.

We study the transcriptional landscape of C. glabrata and identify differentially expressed genes between pH4 and ph8 as well as between nitrosative stress (GSNO) treatment and controll. Candida glabrata was grown in standard nutrient-rich medium, ph4, ph8 and in GSNO conditions. Paired-end and strand-specific RNA-Seq was performed on three biological replicates after PolyA filtering. A fourth replicate without filtering was sequenced single-end and not strand-specific.

RNA-seq We study the transcriptional landscape of C. glabrata and identify differentially expressed genes between pH4 and ph8 as well as between nitrosative stress (GSNO) treatment and controll. Candida glabrata was grown in standard nutrient-rich medium, ph4, ph8 and in GSNO conditions. Paired-end and strand-specific RNA-Seq was performed on three biological replicates after PolyA filtering. A fourth replicate without filtering was sequenced single-end and not strand-specific.

Experiment Design: ...

Experiment Design: growth_condition_design, co-expression_design Experimental Factor: Compound, Organism, Strain Experimental Factor Type: compound, organism, strain_or_line Quality Control Type: technical_replicate, biological_replicate

Specimen Material Type Organism Strain or Line Organism Part Organism Status Sex Age Age Time Unit Sampling Time Point Sampling Time Point Unit Temperature Temperature Unit pH Atmosphere Compound Media Nutrients Fold Change Assignment Name Fold Change Assignment ...

TANDEM MASS SPECTROMETRY (GC) Xcalibur GUI

Source Specimen Material Type Organism Strain or Line Organism Part Organism Status Sex Age Age Time Unit Sampling Time Point Sampling Time Point Unit Temperature Temperature Unit pH Atmosphere Compound Media Nutrients Fold Change Assignment Name Fold Change Assignment Feature acidiphilium_acidithrix_sup_2 whole_organism Acidiphilium C61 whole culture fresh n/a n/a n/a Acidithrix supernatant acidiphilium_acidithrix_sup_1 whole_organism Acidiphilium C61 ...