GC-MS: Sticking together: Inter-species aggregation of bacteria isolated from iron snow is controlled by chemical signaling

TANDEM MASS SPECTROMETRY (GC) Xcalibur GUI

Source Specimen Material Type Organism Strain or Line Organism Part Organism Status Sex Age Age Time Unit Sampling Time Point Sampling Time Point Unit Temperature Temperature Unit pH Atmosphere Compound Media Nutrients Fold Change Assignment Name Fold Change Assignment Feature acidiphilium_acidithrix_sup_2 whole_organism Acidiphilium C61 whole culture fresh n/a n/a n/a Acidithrix supernatant acidiphilium_acidithrix_sup_1 whole_organism Acidiphilium C61 whole culture fresh n/a n/a n/a Acidithrix supernatant acidithrix_acidiphilium_sup_1 whole_organism Acidithrix C25 whole culture fresh n/a n/a n/a Acidiphilium supernatant acidithrix_acidithrix_sup_1 whole_organism Acidithrix C25 whole culture fresh n/a n/a n/a Acidithrix supernatant acidiphilium_acidiphilium_sup_2 whole_organism Acidiphilium C61 whole culture fresh n/a n/a n/a Acidiphilium supernatant acidithrix_acidiphilium_sup_2 whole_organism Acidithrix C25 whole culture fresh n/a n/a n/a Acidiphilium supernatant acidithrix_acidithrix_sup_2 whole_organism Acidithrix C25 whole culture fresh n/a n/a n/a Acidithrix supernatant acidiphilium_acidiphilium_sup_1 whole_organism Acidiphilium C61 whole culture fresh n/a n/a n/a Acidiphilium supernatant

Extraction Specimen Extract Name Sequence of working steps Protein Amount Amount Unit Volume Volume Unit Label acidiphilium_acidithrix_sup_1 acidiphilium_acidithrix_sup_1_e n/a n/a acidiphilium_acidithrix_sup_2 acidiphilium_acidithrix_sup_2_e n/a n/a acidiphilium_acidiphilium_sup_1 acidiphilium_acidiphilium_sup_1_e n/a n/a acidiphilium_acidiphilium_sup_2 acidiphilium_acidiphilium_sup_2_e n/a n/a acidithrix_acidithrix_sup_1 acidithrix_acidithrix_sup_1_e n/a n/a acidithrix_acidithrix_sup_2 acidithrix_acidithrix_sup_2_e n/a n/a acidithrix_acidiphilium_sup_1 acidithrix_acidiphilium_sup_1_e n/a n/a acidithrix_acidiphilium_sup_2 acidithrix_acidiphilium_sup_2_e n/a n/a

GC-MS/MS Specimen Assay Name GC Instrument MS Mass Detector Type MS Mass Analyser Type MS External Calibration MS Internal Calibration MS Operation Mode MS Mass Range MS Resolution MS Maximum Injection Time MS Time Unit MS Autogain Control Target MS Further Fragmentation Isolisation Width MS Further Fragmentation Collision Energy MS Energy Unit MS Furhter Fragmentation Gas MS2 Mass Range MS2 Resolution MS2 Maximum Injection Time MS2 Time Unit MS2 Autogain Control Target acidiphilium_acidithrix_sup_1 acidiphilium_acidithrix_sup_1_gc Trace 1310 (Thermo Scientific) electron impact triple-quadrupole 50-650 n/a n/a n/a acidiphilium_acidithrix_sup_2 acidiphilium_acidithrix_sup_2_gc Trace 1310 (Thermo Scientific) electron impact triple-quadrupole 50-650 n/a n/a n/a acidiphilium_acidiphilium_sup_1 acidiphilium_acidiphilium_sup_1_gc Trace 1310 (Thermo Scientific) electron impact triple-quadrupole 50-650 n/a n/a n/a acidiphilium_acidiphilium_sup_2 acidiphilium_acidiphilium_sup_2_gc Trace 1310 (Thermo Scientific) electron impact triple-quadrupole 50-650 n/a n/a n/a acidithrix_acidithrix_sup_1 acidithrix_acidithrix_sup_1_gc Trace 1310 (Thermo Scientific) electron impact triple-quadrupole 50-650 n/a n/a n/a acidithrix_acidithrix_sup_2 acidithrix_acidithrix_sup_2_gc Trace 1310 (Thermo Scientific) electron impact triple-quadrupole 50-650 n/a n/a n/a acidithrix_acidiphilium_sup_1 acidithrix_acidiphilium_sup_1_gc Trace 1310 (Thermo Scientific) electron impact triple-quadrupole 50-650 n/a n/a n/a acidithrix_acidiphilium_sup_2 acidithrix_acidiphilium_sup_2_gc Trace 1310 (Thermo Scientific) electron impact triple-quadrupole 50-650 n/a n/a n/a