Defining the transcriptional landscape of Candida glabrata by RNA-Seq

This project aims to gather data and metadata from first funding period projects that have not been continued during the second funding period

We study the transcriptional landscape of C. glabrata and identify differentially expressed genes between pH4 and ph8 as well as between nitrosative stress (GSNO) treatment and controll. Candida glabrata was grown in standard nutrient-rich medium, ph4, ph8 and in GSNO conditions. Paired-end and strand-specific RNA-Seq was performed on three biological replicates after PolyA filtering. A fourth replicate without filtering was sequenced single-end and not strand-specific.

RNA-seq We study the transcriptional landscape of C. glabrata and identify differentially expressed genes between pH4 and ph8 as well as between nitrosative stress (GSNO) treatment and controll. Candida glabrata was grown in standard nutrient-rich medium, ph4, ph8 and in GSNO conditions. Paired-end and strand-specific RNA-Seq was performed on three biological replicates after PolyA filtering. A fourth replicate without filtering was sequenced single-end and not strand-specific.

Experiment Design: ...