Evgeni Bratovanov

About Evgeni Bratovanov:
No description specified

SEEK ID: https://data.chembiosys.de/people/40

Location:  Germany

Expertise: Molecular Biology, Microbiology, Microscopy, Biotechnology, molecular cloning

Tools: Genetic modification, PCR, heterologous expression

ORCID: https://orcid.org/0000-0002-6954-7376

help Their tags

This person has not yet tagged anything.

Related items

B1

Cryptic Mediators at the Bacterial-Fungal Interface: Mushroom Soft Rot Diseases as Infection Model

Public web page: Not specified

Total ChemBioSys
No description specified

Public web page: Not specified

RDM training

Training space to learn how to use SEEK

Public web page: Not specified

1 hidden item
Multimodal Molecular Imaging and Identification of Bacterial Toxins Causing Mushroom Soft Rot and Cavity Disease
Z02

Abstract (Expand)

Soft rot disease of edible mushrooms leads to rapid degeneration of fungal tissue and thus severely affects farming productivity worldwide. The bacterial mushroom pathogen Burkholderia gladioli pv. … agaricicola has been identified as the cause. Yet, little is known about the molecular basis, the spatial distribution and the biological role of antifungal agents and toxins involved in this infectious disease. We combine genome mining, metabolic profiling, MALDI-Imaging and UV Raman spectroscopy, to detect, identify and visualize a complex of chemical mediators and toxins produced by the pathogen during the infection process, including toxoflavin, caryoynencin, and sinapigladioside. Furthermore, targeted gene knockouts and in vitro assays link antifungal agents to prevalent symptoms of soft rot, mushroom browning, and impaired mycelium growth. Comparisons of related pathogenic, mutualistic and environmental Burkholderia spp. indicate that the arsenal of antifungal agents may have paved the way for ancestral bacteria to colonize niches where frequent, antagonistic interactions with fungi occur. Our findings not only demonstrate the power of label-free, in vivo detection of polyyne virulence factors by Raman imaging, but may also inspire new approaches to disease control.

Authors: Benjamin Dose, Tawatchai Thongkongkaew, David Zopf, Hak Joong Kim, Evgeni V. Bratovanov, María García-Altares Pérez, Kirstin Scherlach, Jana Krabbe, Claudia Ross, Ron Hermenau, Sarah P. Niehs, Anja Silge, Julian Hniopek, Michael Schmitt, Jürgen Popp, Christian Hertweck

Date Published: 7th Jul 2021

Publication Type: Journal

6 hidden items
Powered by
 (v.1.11.0)
About | ChemBioSys | Funding and Programmes | Credits
Copyright © 2008 - 2021 The University of Manchester and HITS gGmbH