Sarah Niehs

About Sarah Niehs:
No description specified

SEEK ID: https://data.chembiosys.de/people/111

Location:  Germany

ORCID: https://orcid.org/0000-0002-2012-5949

Joined: 21st Sep 2020

Expertise: Structure Elucidation, Fermentation, Microbiology, Chemical Ecology

Tools: MS/MS, LC/MS, Fermentation, NMR

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B1

Cryptic Mediators at the Bacterial-Fungal Interface: Mushroom Soft Rot Diseases as Infection Model

Public web page: Not specified

Total ChemBioSys
No description specified

Public web page: Not specified

Food-poisoning Bacteria and their Chemical Arsenal
Total ChemBioSys, B1

Burkholderia gladioli, cocovenenans, food-poisoning, tempeh, bonkrekic, acid, chemical, Natural Products

Submitter: Sarah Niehs

Studies: No Studies

Assays: No Assays

Chemical Ecology of Insect Symbionts
B1

Natural Products, Structure Elucidation, Burkholderia, gladioli, Lagria, villosa, insect symbiont, protective symbiosis

Submitter: Sarah Niehs

Studies: No Studies

Assays: No Assays

Natural Products of Endofungal Symbionts (Rhizopus-Mycetohabitans Interaction)
B1

Natural Products, Endosymbionts, Rhizopus microsporus, Mycetohabitans, Structure Elucidation, Symbiosis

Submitter: Sarah Niehs

Studies: No Studies

Assays: No Assays

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NMR data sinapigladioside congener
Total ChemBioSys, B1

NMR data sinapigladioside congener

Creator: Sarah Niehs

Submitter: Sarah Niehs

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NMR data bolagladin B
Total ChemBioSys, B1
No description specified

Creator: Sarah Niehs

Submitter: Sarah Niehs

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NMR data gladiofungin A
B1
No description specified

Creator: Sarah Niehs

Submitter: Sarah Niehs

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NMR data necroxime A
B1
No description specified

Creator: Sarah Niehs

Submitter: Sarah Niehs

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NMR data endopyrrole A
B1
No description specified

Creator: Sarah Niehs

Submitter: Sarah Niehs

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Multimodal Molecular Imaging and Identification of Bacterial Toxins Causing Mushroom Soft Rot and Cavity Disease
Z02

Abstract (Expand)

Soft rot disease of edible mushrooms leads to rapid degeneration of fungal tissue and thus severely affects farming productivity worldwide. The bacterial mushroom pathogen Burkholderia gladioli pv. … agaricicola has been identified as the cause. Yet, little is known about the molecular basis, the spatial distribution and the biological role of antifungal agents and toxins involved in this infectious disease. We combine genome mining, metabolic profiling, MALDI-Imaging and UV Raman spectroscopy, to detect, identify and visualize a complex of chemical mediators and toxins produced by the pathogen during the infection process, including toxoflavin, caryoynencin, and sinapigladioside. Furthermore, targeted gene knockouts and in vitro assays link antifungal agents to prevalent symptoms of soft rot, mushroom browning, and impaired mycelium growth. Comparisons of related pathogenic, mutualistic and environmental Burkholderia spp. indicate that the arsenal of antifungal agents may have paved the way for ancestral bacteria to colonize niches where frequent, antagonistic interactions with fungi occur. Our findings not only demonstrate the power of label-free, in vivo detection of polyyne virulence factors by Raman imaging, but may also inspire new approaches to disease control.

Authors: Benjamin Dose, Tawatchai Thongkongkaew, David Zopf, Hak Joong Kim, Evgeni V. Bratovanov, María García-Altares Pérez, Kirstin Scherlach, Jana Krabbe, Claudia Ross, Ron Hermenau, Sarah P. Niehs, Anja Silge, Julian Hniopek, Michael Schmitt, Jürgen Popp, Christian Hertweck

Date Published: 7th Jul 2021

Publication Type: Journal

Biosynthesis of Sinapigladioside, an Antifungal Isothiocyanate from Burkholderia Symbionts.
Total ChemBioSys, B1

Abstract (Expand)

Sinapigladioside is a rare isothiocyanate-bearing natural product from beetle-associated bacteria ( Burkholderia gladioli ) that may protect the beetle offspring against entomopathogenic fungi. The … biosynthetic origin of sinapigladioside has been elusive, and little is known about bacterial isothiocyanate biosynthesis in general. On the basis of stable-isotope labeling, bioinformatics, and mutagenesis we identified the sinapigladioside biosynthesis gene cluster in the symbiont and found that an isonitrile synthase plays a key role in the biosynthetic pathway. Genome mining and network analyses indicate that related gene clusters are distributed across various bacterial phyla including producers of both nitriles and isothiocyanates. Our findings support a model for bacterial isothiocyanate biosynthesis by sulfur transfer onto isonitrile precursors.

Authors: B. Dose, S. P. Niehs, K. Scherlach, S. Shahda, L. V. Florez, M. Kaltenpoth, C. Hertweck

Date Published: 19th Mar 2021

Publication Type: Journal

Genomics-Driven discovery of natural products from symbiotic Burkholderia species
B1

Abstract

Not specified

Editor:

Date Published: 20th Nov 2020

Publication Type: Phd Thesis

Food-Poisoning Bacteria Employ a Citrate Synthase and a Type II NRPS To Synthesize Bolaamphiphilic Lipopeptide Antibiotics.
Total ChemBioSys, B1

Abstract (Expand)

Mining the genome of the food-spoiling bacterium Burkholderia gladioli pv. cocovenenans revealed five nonribosomal peptide synthetase (NRPS) gene clusters, including an orphan gene locus (bol). Gene … inactivation and metabolic profiling linked the bol gene cluster to novel bolaamphiphilic lipopeptides with antimycobacterial activity. A combination of chemical analysis and bioinformatics elucidated the structures of bolagladin A and B, lipocyclopeptides featuring an unusual dehydro-beta-alanine enamide linker fused to an unprecedented tricarboxylic fatty acid tail. Through a series of targeted gene deletions, we proved the involvement of a designated citrate synthase (CS), priming ketosynthases III (KS III), a type II NRPS, including a novel desaturase for enamide formation, and a multimodular NRPS in generating the cyclopeptide. Network analyses revealed the evolutionary origin of the CS and identified cryptic CS/NRPS gene loci in various bacterial genomes.

Authors: B. Dose, C. Ross, S. P. Niehs, K. Scherlach, J. P. Bauer, C. Hertweck

Date Published: 11th Aug 2020

Publication Type: Not specified

Insect-Associated Bacteria Assemble the Antifungal Butenolide Gladiofungin by Non-Canonical Polyketide Chain Termination.
Total ChemBioSys, B1

Abstract (Expand)

Genome mining of one of the protective symbionts ( Burkholderia gladioli ) of the invasive beetle Lagria villosa revealed a cryptic gene cluster coding for the biosynthesis of a novel antifungal … polyketide with a glutarimide pharmacophore. Targeted gene inactivation, metabolic profiling and bioassays led to the discovery of the gladiofungins as yet overlooked components of the antimicrobial armory of the beetle symbiont, specifically against the entomopathogenic fungus Purpureocillium lilacinum . By mutational analyses, isotope labeling and in silico analyses of the modular polyketide synthase, we found that the rare butenolide moiety of gladiofungins derives from an unprecedented polyketide chain termination reaction involving a glycerol-derived C3 building block. The key role of an A-factor synthase (AfsA)-like offloading domain was corroborated by CRISPR-Cas-mediated gene editing, which facilitated the precise excision within a PKS domain.

Authors: S. P. Niehs, J. Kumpfmuller, B. Dose, R. F. Little, K. Ishida, L. V. Florez, M. Kaltenpoth, C. Hertweck

Date Published: 26th Jun 2020

Publication Type: Not specified

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