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Sarah Niehs

About Sarah Niehs:
No description specified

SEEK ID: https://data.chembiosys.de/people/111

Location:  Germany

Expertise: Structure Elucidation, Fermentation, Microbiology, Chemical Ecology

Tools: MS/MS, LC/MS, Fermentation, NMR

ORCID: https://orcid.org/0000-0002-2012-5949

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B1

Cryptic Mediators at the Bacterial-Fungal Interface: Mushroom Soft Rot Diseases as
Infection Model

Public web page: Not specified

Total ChemBioSys
No description specified

Public web page: Not specified

Food-poisoning Bacteria and their Chemical Arsenal
Total ChemBioSys, B1

Burkholderia gladioli, cocovenenans, food-poisoning, tempeh, bonkrekic, acid, chemical, Natural Products

Snapshots: No snapshots

Chemical Ecology of Insect Symbionts
B1

Natural Products, Structure Elucidation, Burkholderia, gladioli, Lagria, villosa, insect symbiont, protective symbiosis

Snapshots: No snapshots

Natural Products of Endofungal Symbionts (Rhizopus-Mycetohabitans Interaction)
B1

Natural Products, Endosymbionts, Rhizopus microsporus, Mycetohabitans, Structure Elucidation, Symbiosis

Snapshots: No snapshots

1 hidden item
22 hidden items
NMR data sinapigladioside congener
Total ChemBioSys, B1

NMR data sinapigladioside congener

Creator: Sarah Niehs

Submitter: Sarah Niehs

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NMR data bolagladin B
Total ChemBioSys, B1
No description specified

Creator: Sarah Niehs

Submitter: Sarah Niehs

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NMR data gladiofungin A
B1
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Creator: Sarah Niehs

Submitter: Sarah Niehs

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NMR data necroxime A
B1
No description specified

Creator: Sarah Niehs

Submitter: Sarah Niehs

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NMR data endopyrrole A
B1
No description specified

Creator: Sarah Niehs

Submitter: Sarah Niehs

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Biosynthesis of Sinapigladioside, an Antifungal Isothiocyanate from Burkholderia Symbionts.
Total ChemBioSys, B1

Abstract (Expand)

Sinapigladioside is a rare isothiocyanate-bearing natural product from beetle-associated bacteria ( Burkholderia gladioli ) that may protect the beetle offspring against entomopathogenic fungi. The … biosynthetic origin of sinapigladioside has been elusive, and little is known about bacterial isothiocyanate biosynthesis in general. On the basis of stable-isotope labeling, bioinformatics, and mutagenesis we identified the sinapigladioside biosynthesis gene cluster in the symbiont and found that an isonitrile synthase plays a key role in the biosynthetic pathway. Genome mining and network analyses indicate that related gene clusters are distributed across various bacterial phyla including producers of both nitriles and isothiocyanates. Our findings support a model for bacterial isothiocyanate biosynthesis by sulfur transfer onto isonitrile precursors.

Authors: B. Dose, S. P. Niehs, K. Scherlach, S. Shahda, L. V. Florez, M. Kaltenpoth, C. Hertweck

Date Published: 19th Mar 2021

Publication Type: Journal

Genomics-Driven discovery of natural products from symbiotic Burkholderia species
B1

Abstract

Not specified

Editor:

Date Published: 20th Nov 2020

Publication Type: Phd Thesis

Food-Poisoning Bacteria Employ a Citrate Synthase and a Type II NRPS To Synthesize Bolaamphiphilic Lipopeptide Antibiotics.
Total ChemBioSys, B1

Abstract (Expand)

Mining the genome of the food-spoiling bacterium Burkholderia gladioli pv. cocovenenans revealed five nonribosomal peptide synthetase (NRPS) gene clusters, including an orphan gene locus (bol). Gene … inactivation and metabolic profiling linked the bol gene cluster to novel bolaamphiphilic lipopeptides with antimycobacterial activity. A combination of chemical analysis and bioinformatics elucidated the structures of bolagladin A and B, lipocyclopeptides featuring an unusual dehydro-beta-alanine enamide linker fused to an unprecedented tricarboxylic fatty acid tail. Through a series of targeted gene deletions, we proved the involvement of a designated citrate synthase (CS), priming ketosynthases III (KS III), a type II NRPS, including a novel desaturase for enamide formation, and a multimodular NRPS in generating the cyclopeptide. Network analyses revealed the evolutionary origin of the CS and identified cryptic CS/NRPS gene loci in various bacterial genomes.

Authors: B. Dose, C. Ross, S. P. Niehs, K. Scherlach, J. P. Bauer, C. Hertweck

Date Published: 11th Aug 2020

Publication Type: Not specified

Insect-Associated Bacteria Assemble the Antifungal Butenolide Gladiofungin by Non-Canonical Polyketide Chain Termination.
Total ChemBioSys, B1

Abstract (Expand)

Genome mining of one of the protective symbionts ( Burkholderia gladioli ) of the invasive beetle Lagria villosa revealed a cryptic gene cluster coding for the biosynthesis of a novel antifungal … polyketide with a glutarimide pharmacophore. Targeted gene inactivation, metabolic profiling and bioassays led to the discovery of the gladiofungins as yet overlooked components of the antimicrobial armory of the beetle symbiont, specifically against the entomopathogenic fungus Purpureocillium lilacinum . By mutational analyses, isotope labeling and in silico analyses of the modular polyketide synthase, we found that the rare butenolide moiety of gladiofungins derives from an unprecedented polyketide chain termination reaction involving a glycerol-derived C3 building block. The key role of an A-factor synthase (AfsA)-like offloading domain was corroborated by CRISPR-Cas-mediated gene editing, which facilitated the precise excision within a PKS domain.

Authors: S. P. Niehs, J. Kumpfmuller, B. Dose, R. F. Little, K. Ishida, L. V. Florez, M. Kaltenpoth, C. Hertweck

Date Published: 26th Jun 2020

Publication Type: Not specified

Mining Symbionts of a Spider-Transmitted Fungus Illuminates Uncharted Biosynthetic Pathways to Cytotoxic Benzolactones
Total ChemBioSys, B1

Abstract (Expand)

A spider-transmitted fungus (Rhizopus microspo- rus) that was isolated from necrotic human tissue was found to harbor endofungal bacteria (Burkholderia sp.). Metabolic profiling of the symbionts revealed … a complex of cytotoxic agents (necroximes). Their structures were characterized as oxime-substituted benzolactone enamides with a peptidic side chain. The potently cytotoxic necroximes are also formed in symbiosis with the fungal host and could have contributed to the necrosis. Genome sequencing and computational analyses revealed a novel modular PKS/NRPS assembly line equipped with several non-canonical domains. Based on gene-deletion mutants, we propose a biosynthetic model for bacterial benzolactones. We identified specific traits that serve as genetic handles to find related salicylate macrolide pathways (lobata- mide, oximidine, apicularen) in various other bacterial genera. Knowledge of the biosynthetic pathway enables biosynthetic engineering and genome-mining approaches.

Authors: Sarah Niehs, Benjamin Dose, Sophie Richter, Sacha J. Pidot, Timothy P. Stinear, Hans-Martin Dahse, Christian Hertweck

Date Published: 10th Feb 2020

Publication Type: Not specified

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