Nina Kreuzenbeck

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About Nina Kreuzenbeck:

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SEEK ID: https://data.chembiosys.de/people/94

Location: Germany

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Total ChemBioSys

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A06

ISOLATION, CHARACTERIZATION AND FUNCTIONAL ANALYSIS OF NOVEL BIOMOLECULES FROM THE MUTUALISTIC FOOD FUNGUS AND ASSOCIATED MICROBES OF FUNGUS-GROWING TERMITES

Public web page: Not specified

Start date: 1st Jun 2018

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Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute

Country: Germany

City: Jena

Web page: http://www.leibniz-hki.de

Max-Planck-Institut für Chemische Ökologie

Country: Germany

City: Jena

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The Termite Fungal Cultivar Termitomyces Combines Diverse Enzymes and Oxidative Reactions for Plant Biomass Conversion.

A06

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Macrotermitine termites have domesticated fungi in the genus Termitomyces as their primary food source using predigested plant biomass. To access the full nutritional value of lignin-enriched plant … biomass, the termite-fungus symbiosis requires the depolymerization of this complex phenolic polymer. While most previous work suggests that lignocellulose degradation is accomplished predominantly by the fungal cultivar, our current understanding of the underlying biomolecular mechanisms remains rudimentary. Here, we provide conclusive omics and activity-based evidence that Termitomyces employs not only a broad array of carbohydrate-active enzymes (CAZymes) but also a restricted set of oxidizing enzymes (manganese peroxidase, dye decolorization peroxidase, an unspecific peroxygenase, laccases, and aryl-alcohol oxidases) and Fenton chemistry for biomass degradation. We propose for the first time that Termitomyces induces hydroquinone-mediated Fenton chemistry (Fe(2+) + H2O2 + H(+) --> Fe(3+) + (*)OH + H2O) using a herein newly described 2-methoxy-1,4-dihydroxybenzene (2-MH2Q, compound 19)-based electron shuttle system to complement the enzymatic degradation pathways. This study provides a comprehensive depiction of how efficient biomass degradation by means of this ancient insect's agricultural symbiosis is accomplished. IMPORTANCE Fungus-growing termites have optimized the decomposition of recalcitrant plant biomass to access valuable nutrients by engaging in a tripartite symbiosis with complementary contributions from a fungal mutualist and a codiversified gut microbiome. This complex symbiotic interplay makes them one of the most successful and important decomposers for carbon cycling in Old World ecosystems. To date, most research has focused on the enzymatic contributions of microbial partners to carbohydrate decomposition. Here, we provide genomic, transcriptomic, and enzymatic evidence that Termitomyces also employs redox mechanisms, including diverse ligninolytic enzymes and a Fenton chemistry-based hydroquinone-catalyzed lignin degradation mechanism, to break down lignin-rich plant material. Insights into these efficient decomposition mechanisms reveal new sources of efficient ligninolytic agents applicable for energy generation from renewable sources.

Authors: F. Schalk, C. Gostincar, N. B. Kreuzenbeck, B. H. Conlon, E. Sommerwerk, P. Rabe, I. Burkhardt, T. Kruger, O. Kniemeyer, A. A. Brakhage, N. Gunde-Cimerman, Z. W. de Beer, J. S. Dickschat, M. Poulsen, C. Beemelmanns

Date Published: 15th Jun 2021

Publication Type: Journal

PubMed ID: 34126770

Citation: mBio. 2021 Jun 15:e0355120. doi: 10.1128/mBio.03551-20.

Created: 28th Jun 2021 at 10:44

Targeted Discovery of Tetrapeptides and Cyclic Polyketide-Peptide Hybrids from a Fungal Antagonist of Farming Termites.

A06

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Herein, we report the targeted isolation and characterization of four linear nonribosomally synthesized tetrapeptides (pseudoxylaramide A-D) and two cyclic nonribosomal peptide synthetase-polyketide …

Authors: F. Schalk, S. Um, H. Guo, N. B. Kreuzenbeck, H. Gorls, Z. W. de Beer, C. Beemelmanns

Date Published: 15th Oct 2020

Publication Type: Journal

PubMed ID: 32470183

Citation: Chembiochem. 2020 Oct 15;21(20):2991-2996. doi: 10.1002/cbic.202000331. Epub 2020 Jul 2.

Created: 3rd Feb 2021 at 15:58

Disease-free monoculture farming by fungus-growing termites.

A06

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Fungus-growing termites engage in an obligate mutualistic relationship with Termitomyces fungi, which they maintain in monocultures on specialised fungus comb structures, without apparent problems with …

Authors: S. Otani, V. L. Challinor, N. B. Kreuzenbeck, S. Kildgaard, S. Krath Christensen, L. L. M. Larsen, D. K. Aanen, S. A. Rasmussen, C. Beemelmanns, M. Poulsen

Date Published: 19th Jun 2019

Publication Type: Journal

PubMed ID: 31217550

Citation: Sci Rep. 2019 Jun 19;9(1):8819. doi: 10.1038/s41598-019-45364-z.

Created: 5th Feb 2021 at 06:49

Mechanistic characterization of three sesquiterpene synthases from the termite-associated fungus Termitomyces.

A06

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Three terpene synthases from the termite associated fungus Termitomyces were functionally characterized as (+)-intermedeol synthase, (-)-gamma-cadinene synthase and (+)-germacrene D-4-ol synthase, with …

Authors: I. Burkhardt, N. B. Kreuzenbeck, C. Beemelmanns, J. S. Dickschat

Date Published: 27th Mar 2019

Publication Type: Journal

PubMed ID: 30693926

Citation: Org Biomol Chem. 2019 Mar 27;17(13):3348-3355. doi: 10.1039/c8ob02744g.

Created: 5th Feb 2021 at 06:57

Pseudoxylallemycins A-F, Cyclic Tetrapeptides with Rare Allenyl Modifications Isolated from Pseudoxylaria sp. X802: A Competitor of Fungus-Growing Termite Cultivars.

B1, A06

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Based on fungus-fungus pairing assays and HRMS-based dereplication strategy, six new cyclic tetrapeptides, pseudoxylallemycins A-F (1-6), were isolated from the termite-associated fungus Pseudoxylaria …

Authors: H. Guo, N. B. Kreuzenbeck, S. Otani, M. Garcia-Altares, H. M. Dahse, C. Weigel, D. K. Aanen, C. Hertweck, M. Poulsen, C. Beemelmanns

Date Published: 25th Jun 2016

Publication Type: Not specified

PubMed ID: 27341414

Citation: Org Lett. 2016 Jul 15;18(14):3338-41. doi: 10.1021/acs.orglett.6b01437. Epub 2016 Jun 24.

Created: 23rd Nov 2017 at 09:18

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