Raman spectroscopy towards clinical application: drug monitoring and pathogen identification

Abstract:

Raman spectroscopy is a label-free method that measures quickly and contactlessly, providing detailed information from the sample, and has proved to be an ideal tool for medical and life science research. In this review, recent advances of the technique towards drug monitoring and pathogen identification by the Jena Research Groups are reviewed. Surface-enhanced Raman spectroscopy (SERS) and ultraviolet resonance Raman spectroscopy in hollow-core optical fibres enable the detection of drugs at low concentrations as shown for the metabolites of the immunosuppressive drug 6-mercaptopurine as well as antimalarial agents. Furthermore, Raman spectroscopy can be used to characterise pathogenic bacteria in infectious diseases directly from body fluids, making time-consuming cultivation processes dispensable. Using the example of urinary tract infection, it is shown how bacteria can be identified from patients' urine samples within <1h. The methods cover both single-cell analysis and dielectrophoretic capturing of bacteria in suspension. The latter method could also be used for fast (<3.5h) identification of antibiotic resistance as shown exemplarily for vancomycin-resistant enterococci.

SEEK ID: https://data.chembiosys.de/publications/19

PubMed ID: 26612228

Projects: Z02

Publication type: Not specified

Journal: Int J Antimicrob Agents

Citation:

Date Published: 6th Nov 2015

Registered Mode: Not specified

Authors: U. Neugebauer, ,

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Created: 29th Feb 2016 at 08:45

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