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Mechanisms of Bacteria-triggered Activation of Fungal Silent Gene Clusters and Impact of Produced Compounds on Microorganisms
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Abstract (Expand)
Targeted HRMS(2)-GNPS-based metabolomic analysis of Pseudoxylaria sp. X187, a fungal antagonist of the fungus-growing termite symbiosis, resulted in the identification of two lipopeptidic congeners of … xylacremolides, named xylacremolide C and D, which are built from d-phenylalanine, l-proline and an acetyl-CoA starter unit elongated by four malonyl-CoA derived ketide units. The putative xya gene cluster was identified from a draft genome generated by Illumina and PacBio sequencing and RNAseq studies. Biological activities of xylacremolide A and B were evaluated and revealed weak histone deacetylase inhibitory (HDACi) and antifungal activities, as well as moderate protease inhibition activity across a panel of nine human, viral and bacterial proteases.
Authors: F. Schalk, J. Fricke, S. Um, B. H. Conlon, H. Maus, N. Jager, T. Heinzel, T. Schirmeister, M. Poulsen, C. Beemelmanns
Date Published: 24th May 2021
Publication Type: Journal
PubMed ID: 34046176
Citation: RSC Adv. 2021 May 24;11(31):18748-18756. doi: 10.1039/d1ra00997d.
Created: 31st May 2021 at 11:27