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Identification of Secondary Metabolites with Roles in Interactions between Chlamydomonas reinhardtii and Other Microorganisms
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Organisms: Chlamydomonas reinhardtii, Mortierella hyalina
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Abstract (Expand)
The exchange of chemical compounds is central to the interactions of microalgae with other microorganisms. Although foundational for many food webs, these interactions have been poorly studied compared … with higher plant-microbe interactions. Emerging insights have begun to reveal how these interactions and the participating chemical compounds shape microbial communities and broadly impact biogeochemical processes.
Date Published: 28th Oct 2015
Publication Type: Not specified
PubMed ID: 26522386
Citation:
Created: 19th Nov 2015 at 12:43, Last updated: 9th Feb 2023 at 08:34
Abstract (Expand)
Polyketide synthases (PKSs) occur in many bacteria, fungi and plants. They are highly versatile enzymes involved in the biosynthesis of a large variety of compounds including antimicrobial agents, … polymers associated with bacterial cell walls and plant pigments. While harmful algae are known to produce polyketide toxins, sequences of the genomes of non-toxic algae, including those of many green-algal species, have surprisingly revealed the presence of genes encoding type I PKSs. The genome of the model alga Chlamydomonas reinhardtii (Chlorophyta) contains a single type I PKS gene, designated PKS1 (Cre10.g449750), which encodes a giant PKS with a predicted mass of 2.3 MDa. Here, we show that PKS1 is induced in two-day old zygotes and is required for their development into zygospores, the dormant stage of the zygote. Wild-type zygospores contain knob-like structures (~50 nm diameter) that form at the cell surface and develop a central cell wall layer; both of these structures are absent from homozygous pks1 mutants. Additionally, in contrast to wild-type zygotes, chlorophyll degradation is delayed in homozygous pks1 mutant zygotes, indicating a disruption of zygospore development. In agreement with a role of the PKS in the formation of the highly resistant zygospore wall, mutant zygotes have lost the formidable desiccation tolerance of wild-type zygotes. Together, our results represent functional analyses of a PKS mutant in a photosynthetic eukaryotic microorganism, revealing a central function for polyketides in the sexual cycle and survival under stressful environmental conditions. This article is protected by copyright. All rights reserved.
Authors: N. Heimerl, E. Hommel, M. Westermann, D. Meichsner, M. Lohr, C. Hertweck, A. R. Grossman, M. Mittag, S. Sasso
Date Published: No date defined
Publication Type: Not specified
PubMed ID: 29729034
Citation: Plant J. 2018 May 4. doi: 10.1111/tpj.13948.
Created: 30th May 2018 at 14:07