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3 Publications visible to you, out of a total of 3
Polyhalogenation of Isoflavonoids by the Termite-Associated Actinomadura sp. RB99.

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Based on high-resolution tandem mass spectrometry (HR-MS(2)) and global natural products social molecular networking (GNPS), we found that plant-derived daidzein and genistein derivatives are polyhalogenated … by termite-associated Actinomadura species RB99. MS-guided purification from extracts of bacteria grown under optimized conditions led to the isolation of eight polychlorinated isoflavones, including six unreported derivatives, and seven novel polybrominated derivatives, two of which showed antimicrobial activity.

Authors: S. Rak Lee, F. Schalk, J. W. Schwitalla, R. Benndorf, J. Vollmers, A. K. Kaster, Z. W. de Beer, M. Park, M. J. Ahn, W. H. Jung, C. Beemelmanns, K. H. Kim

Date Published: 23rd Oct 2020

Publication Type: Journal

Streptomyces smaragdinus sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis.

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The taxonomic position of a novel aerobic, Gram-positive actinobacteria, designated strain RB5(T), was determined using a polyphasic approach. The strain, isolated from the gut of the fungus-farming … termite Macrotermes natalensis, showed morphological, physiological and chemotaxonomic properties typical of the genus Streptomyces. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbour of RB5(T) was Streptomyces polyrhachis DSM 42102(T) (98.87 %). DNA-DNA hybridization experiments between strain RB5(T) and S. polyrhachis DSM 42102(T) resulted in a value of 27.4 % (26.8 %). The cell wall of strain RB5(T) contained ll-diaminopimelic acid as the diagnostic amino acid. Mycolic acids and diagnostic sugars in whole-cell hydrolysates were not detected. The strain produced the following major phospholipids: diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and phosphatidylserine. The menaquinone profile showed hexa- and octahydrogenated menaquinones containing nine isoprene units [MK-9(H6) and MK-9(H8)]. The strain exhibited a fatty acid profile containing the following major fatty acids: 12-methyltridecanoic acid (iso-C14 : 0) 12-methyltetradecanoic acid (anteiso-C15 : 0), 13-methyltetradecanoic acid (iso-C15 : 0) and 14-methylpentadecanoic acid (iso-C16 : 0). Here, we propose a novel species of the genus Streptomyces - Streptomyces smaragdinus with the type strain RB5(T) (=VKM Ac-2839(T)=NRRL B65539(T)).

Authors: J. W. Schwitalla, R. Benndorf, K. Martin, J. Vollmers, A. K. Kaster, Z. W. de Beer, M. Poulsen, C. Beemelmanns

Date Published: 25th Sep 2020

Publication Type: Journal

Nocardia macrotermitis sp. nov. and Nocardia aurantia sp. nov., isolated from the gut of the fungus-growing termite Macrotermes natalensis.

Abstract (Expand)

The taxonomic positions of two novel aerobic, Gram-stain-positive Actinobacteria, designated RB20(T) and RB56(T), were determined using a polyphasic approach. Both were isolated from the fungus-farming … termite Macrotermes natalensis. Results of 16S rRNA gene sequence analysis revealed that both strains are members of the genus Nocardia with the closest phylogenetic neighbours Nocardia miyunensis JCM12860(T) (98.9 %) and Nocardia nova DSM44481(T) (98.5 %) for RB20(T) and Nocardia takedensis DSM 44801(T) (98.3 %), Nocardia pseudobrasiliensis DSM 44290(T) (98.3 %) and Nocardia rayongensis JCM 19832(T) (98.2 %) for RB56(T). Digital DNA-DNA hybridization (DDH) between RB20(T) and N. miyunensis JCM12860(T) and N. nova DSM 44481(T) resulted in similarity values of 33.9 and 22.0 %, respectively. DDH between RB56(T) and N. takedensis DSM44801(T) and N. pseudobrasiliensis DSM44290(T) showed similarity values of 20.7 and 22.3 %, respectively. In addition, wet-lab DDH between RB56(T) and N. rayongensis JCM19832(T) resulted in 10.2 % (14.5 %) similarity. Both strains showed morphological and chemotaxonomic features typical for the genus Nocardia, such as the presence of meso-diaminopimelic acid (A2pm) within the cell wall, arabinose and galactose as major sugar components within whole cell-wall hydrolysates, the presence of mycolic acids and major phospholipids (diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol), and the predominant menaquinone MK-8 (H4, omega-cyclo). The main fatty acids for both strains were hexadecanoic acid (C16 : 0), 10-methyloctadecanoic acid (10-methyl C18 : 0) and cis-9-octadecenoic acid (C18 : 1 omega9c). We propose two novel species within the genus Nocardia: Nocardia macrotermitis sp. nov. with the type strain RB20(T) (=VKM Ac-2841(T)=NRRL B65541(T)) and Nocardia aurantia sp. nov. with the type strain RB56(T) (=VKM Ac-2842(T)=NRRL B65542(T)).

Authors: R. Benndorf, J. W. Schwitalla, K. Martin, Z. W. de Beer, J. Vollmers, A. K. Kaster, M. Poulsen, C. Beemelmanns

Date Published: 21st Aug 2020

Publication Type: Journal

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