Genomics-Driven Discovery of a Symbiont-Specific Cyclopeptide from Bacteria Residing in the Rice Seedling Blight Fungus

Abstract:

The rice seedling blight fungus Rhizopus microsporus harbors
endosymbiotic bacteria (Burkholderia rhizoxinica) that produce
the virulence factor rhizoxin and control host development.
Genome mining indicated a massive inventory of cryptic non-
ribosomal peptide synthetase (NRPS) genes, which have not
yet been linked to any natural products. The discovery and full
characterization of a novel cyclopeptide from endofungal bac-
teria is reported. In silico analysis of an orphan, symbiont-spe-
cific NRPS predicted the structure of a nonribosomal peptide,
which was targeted by LC-MS/MS profiling of wild-type and
engineered null mutants. NMR spectroscopy and chemical deri-
vatization elucidated the structure of the bacterial cyclopep-
tide. Phylogenetic analyses revealed the relationship of starter
C domains for rare N-acetyl-capped peptides. Heptarhizin is
produced under symbiotic conditions in geographically con-
strained strains from the Pacific clade; this indicates a potential
ecological role of the peptide.

SEEK ID: https://data.chembiosys.de/publications/99

DOI: 10.1002/cbic.201800400

Projects: B1, Total ChemBioSys

Publication type: Not specified

Journal: ChemBioChem

Citation: S. P. Niehs, B. Dose, K. Scherlach, M. Roth, C. Hertweck, ChemBioChem, 19, 2167–2172, 2018.

Date Published: 16th Aug 2018

Registered Mode: Not specified

Authors: Sarah Niehs, Benjamin Dose, Kirstin Scherlach, Martin Roth, Christian Hertweck

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Niehs, S. P., Dose, B., Scherlach, K., Roth, M., & Hertweck, C. (2018). Genomics-Driven Discovery of a Symbiont-Specific Cyclopeptide from Bacteria Residing in the Rice Seedling Blight Fungus. ChemBioChem, 19(20), 2167–2172. http://doi.org/10.1002/cbic.201800400
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Created: 23rd Sep 2020 at 14:35

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