Helper bacteria halt and disarm mushroom pathogens by linearizing structurally diverse cyclolipopeptides.

Abstract:

The bacterial pathogen Pseudomonas tolaasii severely damages white button mushrooms by secretion of the pore-forming toxin tolaasin, the main virulence factor of brown blotch disease. Yet, fungus-associated helper bacteria of the genus Mycetocola (Mycetocola tolaasinivorans and Mycetocola lacteus) may protect their host by an unknown detoxification mechanism. By a combination of metabolic profiling, imaging mass spectrometry, structure elucidation, and bioassays, we found that the helper bacteria inactivate tolaasin by linearizing the lipocyclopeptide. Furthermore, we found that Mycetocola spp. impair the dissemination of the pathogen by cleavage of the lactone ring of pseudodesmin. The role of pseudodesmin as a major swarming factor was corroborated by identification and inactivation of the corresponding biosynthetic gene cluster. Activity-guided fractionation of the Mycetocola proteome, matrix-assisted laser desorption/ionization (MALDI) analyses, and heterologous enzyme production identified the lactonase responsible for toxin cleavage. We revealed an antivirulence strategy in the context of a tripartite interaction that has high ecological and agricultural relevance.

SEEK ID: https://data.chembiosys.de/publications/97

PubMed ID: 32868430

Projects: B1, Total ChemBioSys

Publication type: Not specified

Journal: Proc Natl Acad Sci U S A

Citation: Proc Natl Acad Sci U S A. 2020 Aug 31. pii: 2006109117. doi: 10.1073/pnas.2006109117.

Date Published: 31st Aug 2020

Registered Mode: Not specified

Authors: R. Hermenau, S. Kugel, A. J. Komor, C. Hertweck

Help
help Submitter
Activity

Views: 331

Created: 22nd Sep 2020 at 09:33

help Attributions

None

Related items

Powered by
(v.1.11.0)
Copyright © 2008 - 2021 The University of Manchester and HITS gGmbH