Genome Mining Reveals Endopyrroles from a Nonribosomal Peptide Assembly Line Triggered in Fungal-Bacterial Symbiosis


The bacterial endosymbiont (Burkholderia rhizoxinica) of the rice
seedling blight fungus (Rhizopus microsporus) harbors a large number of cryptic
biosynthesis gene clusters. Genome mining and sequence similarity networks based
on an encoded nonribosomal peptide assembly line and the associated pyrrole-
forming enzymes in the symbiont indicated that the encoded metabolites are unique
among a large number of tentative pyrrole natural products in diverse and unrelated
bacterial phyla. By performing comparative metabolic pro fi ling using a mutant
generated with an improved pheS Burkholderia counterselection marker, we found
that the symbionts ’ biosynthetic pathway is mainly activated under salt stress and
exclusively in symbiosis with the fungal host. The cryptic metabolites were fully
characterized as novel pyrrole-substituted depsipeptides (endopyrroles). A broader
survey showed that endopyrrole production is a hallmark of geographically distant
endofungal bacteria, which produce the peptides solely under symbiotic conditions.


DOI: 10.1021/acschembio.9b00406

Projects: B1, Total ChemBioSys

Publication type: Not specified

Journal: ACS Chem. Biol.

Citation: S. P. Niehs, B. Dose, K. Scherlach, S. J. Pidot, T. P. Stinear, C. Hertweck

Date Published: 8th Jul 2019

Registered Mode: Not specified

Authors: Sarah Niehs, Benjamin Dose, Kirstin Scherlach, Sacha J. Pidot, Timothy P. Stinear, Christian Hertweck

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Niehs, S. P., Dose, B., Scherlach, K., Pidot, S. J., Stinear, T. P., & Hertweck, C. (2019). Genome Mining Reveals Endopyrroles from a Nonribosomal Peptide Assembly Line Triggered in Fungal–Bacterial Symbiosis. ACS Chemical Biology, 14(8), 1811–1818.

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Created: 23rd Sep 2020 at 14:43

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